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Bioprocess optimization typically involves three major tasks: strain selection, media formulation, and selection of critical process parameters. Strain selection is to select the best producing colony from an early stage culture. Media formulation is developed to find the best combination of different nutrients such as sugars, amino acids, vitamins, minerals, and hormones. Because there are so many different nutrients, the combinational possibilities are enormous. Selection of critical process parameters is done to identify the ideal conditions and the allowable range for each variable. Some of the parameters are pH, dissolved oxygen (DO), feeding strategy, culture temperature, seeding cell density, induction time, and harvest time. In each of the above three tasks, a large number of experiments under varying conditions have to be conducted in order to determine the best colony or the optimal environmental and nutritional conditions.
我的翻译:
一般而言,生物过程优化涉及三个主要任务:菌株、培养基组成和过程关键参数的筛选。菌株筛选目的是从早期的培养过程中选取具有最好生产能力的菌落;培养基优化的目的是找到不同营养组分(如糖、氨基酸、维生素、无机盐和激素)的最佳搭配。因为存在多种不同的营养成分,因此培养基组成的可能性非常巨大。关键过程参数筛选的目的是寻找最合适的条件和每个参数的可允许的范围。参数包括pH,DO,补料策略,培养温度,接种密度,诱导时间和收获时间等。要完成上述三个任务的任何一个都要在不同的条件下进行大量的试验。 |
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