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以下是灭菌注意事项。请大家参与翻译。我们根据翻译的水平和多少送经验值。
Before starting sterilization, verify that all fittings are mounted accurately and tight.You may perform a pressure hold test prior to the sterilization, in order to verify that the culture vessel and connected fittings and peripheral assemblies are airtight and to avoid contamination of the culture medium after sterilization. During such a test do not exceed the max. pressure admissible for the component parts and fittings. The culture vessel is exposed to overpressure during sterilization. Fittings which are not installed properly and fixed tight can be pushed out of their ports. Hot media can be ejected abruptly and can endanger safety or health of exposed persons. During sterilization take care not to touch the heated component parts unintendly (vessel, vessel equipment, pipings). Wear protective gloves if you need to operate
steam connections, manual valves or other hot fittings during sterilization. During the sterilization ensure that unauthorized personnel cannot manipulate the fermentor. You may isolate the hazardous area and place a suitable warning sign. Initiate the sterilization sequence by starting the sterilization program of the control unit. This provides that all automatic safety devices are switched as required. Except
for necessary manual operations during the sequence, as indicated, and for stopping the sequence no measure effecting the sterilization program is allowed. At the end of the sterilization sequence or if the sterilization must be interrupted, wait until the system reaches a safe state (i.e. the culture vessel and all fittings which are heated and pressurized during the sequence, must become pressureless
and be cooled down to ambient temperature) before performing further operations. |
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