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发表于 2008-7-1 20:03:25 | 显示全部楼层 |阅读模式
网站目前需要经验值的地方越来越多了,我们会逐渐开辟新途径,使需要经验值的会员快速得到,所需要的经验值。
以下是灭菌注意事项。请大家参与翻译。我们根据翻译的水平和多少送经验值。
Before starting sterilization, verify that all fittings are mounted accurately and tight.You may perform a pressure hold test prior to the sterilization, in order to verify that the culture vessel and connected fittings and peripheral assemblies are airtight and to avoid contamination of the culture medium after sterilization. During such a test do not exceed the max. pressure admissible for the component parts and fittings. The culture vessel is exposed to overpressure during sterilization. Fittings which are not installed properly and fixed tight can be pushed out of their ports. Hot media can be ejected abruptly and can endanger safety or health of exposed persons. During sterilization take care not to touch the heated component parts unintendly (vessel, vessel equipment, pipings). Wear protective gloves if you need to operate
steam connections, manual valves or other hot fittings during sterilization. During the sterilization ensure that unauthorized personnel cannot manipulate the fermentor. You may isolate the hazardous area and place a suitable warning sign. Initiate the sterilization sequence by starting the sterilization program of the control unit. This provides that all automatic safety devices are switched as required. Except
for necessary manual operations during the sequence, as indicated, and for stopping the sequence no measure effecting the sterilization program is allowed. At the end of the sterilization sequence or if the sterilization must be interrupted, wait until the system reaches a safe state (i.e. the culture vessel and all fittings which are heated and pressurized during the sequence, must become pressureless
and be cooled down to ambient temperature) before performing further operations.
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发表于 2008-7-6 17:13:25 | 显示全部楼层
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发表于 2008-7-7 22:57:34 | 显示全部楼层
好东西啊 希望楼主能够多找些专外的文章 谢谢啊
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发表于 2010-1-21 10:52:10 | 显示全部楼层
灭菌开始前先确保各设备部件已准确稳固安装。为确保罐体及其连接设备和周边各系统的气密性良好并防止灭菌后培养基染菌,可以在灭菌前进行保压测试。在进行该测试操作时不要超出各个设备结构的最大可耐受压力。由于灭菌过程中罐体内部是暴露在超压下的,因此任何安装不当或着安装不紧实的设备均有脱落的可能。高温培养基极有可能喷出,直接威胁操作人员的安全。灭菌过程中一定要注意不要无意间触碰正在加热的设备(罐体、罐体设备和管道)。若确实需要对蒸汽管道、阀门及其他高温设备进行操作则必须佩戴防护手套。灭菌过程中严禁未经批准的人员对发酵罐进行操作。可以对危险区域进行隔离或者设置适当的警示标志。启动控制单元的灭菌程序开始灭菌步骤以确保各安全设备按要求自动开启。除了必要的操作步骤以及停止灭菌外,禁止一切能影响灭菌程序的操作。灭菌接近结束或因其他原因需要中止灭菌时,必须待整个系统达到安全状态(比如培养基温度降至室温)时才可进行进一步操作。
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