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发表于 2006-11-30 12:34:52
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"OUR和CER是什么参数？怎么控制请楼主给详细说一下！"
让我这个外行解释一下, 不对请纠证.
Off-gas from all fermenters can be analyzed by mass spectrometer, enabling the calculation of oxygen uptake rate (OUR), carbon dioxide evolution rate (CER).
每一种微生物发酵都有自己特定的CER, OUR, 我们用它来
(1)判断是否有污染
(2)种子何时转移
(3)feeding control
(4)发酵收获
有很多文献, 如:
Biotechnology and Bioengineering
Volume 21, Issue 7 , Pages 1239 - 1249
On-line identification of parameters in a fermentation process
J. P. Bravard, M. Cordonnier, J. P. Kernevez, J. M. Lebeault
University de Technologie de Compiègne, BP 233 60206, Compiègne, France
Abstract
In a fermentation process, the establishment of gas mass balances provides valuable information and allows both measurements concerning the characteristics of the biomass itself and the monitoring of a cultivation process. If the quantity and oxidation level of substances excreted into the fermentation broth are known or constant, the yield factor and the dry cell-weight production are stoichiometrically related to the quantity of CO2 evolved and to the quantity of O2 consumed. Where frequent measurements of both yield factor and dry cell-weight production are desirable or where rapid adjustment of the parameters is necessary, on-line identification of these parameters is required, An algorithm allowing the identification of the specific growth rate is presented. Moreover, this technique allows one to estimate the percent protein in the biomass during continuous culture. |
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发表于 2006-11-29 21:27:38
发表于 2006-11-30 08:59:45
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