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发表于 2013-9-21 18:46:24
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一株黑曲霉(Aspergillus niger)对孟加拉红和水溶性色浆的脱色特性研究
Decoloring properties of Aspergillus niger to rose bengal and water-soluble color paste
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【作者】 魏宝阳; 高国赋; 田云; 卢向阳; 曹亮; 李顺祥;
【Author】 WEI Baoyang1,GAO Guofu2,TIAN Yun1,3,LU Xiangyang1,3,CAO Liang4,LI Shunxiang1,41. College of Life Science and Technology,Hunan Agricultural University,Changsha 4101282. Sci-tec Information Institution,Hunan Academy of Agricultural Sciences,Changsha 4101253. Hunan Agricultural Bioengineering Research Institute,Changsha 4101284. School of Pharmacy,Hunan University of Traditional Chinese Medicine,Changsha 410208
【机构】 湖南农业大学生物科学技术学院; 湖南省农业科学院科技情报研究所; 湖南省农业生物工程研究所; 湖南中医药大学中药现代化教育厅重点实验室;
【摘要】 以从土壤中分离得到的黑曲霉(Aspergillus niger)菌株为研究对象,采用液体培养的方法,研究其在不同碳源、氮源、起始pH值、金属离子种类及浓度条件下对孟加拉红和水溶性色浆的脱色效果.结果表明,在以豆芽汁培养基为基本培养基、甘油为碳源、(NH4)2SO4为氮源、初始pH值为6.0~8.0条件下的脱色效果最佳,Cu2+对菌体脱色有很强的抑制作用.根据活菌体和死菌体脱色能力的比较,结合菌体干重与脱色能力的相关性分析,初步推测该菌的脱色机理是菌丝吸附染料后,在菌丝体内对其进行降解;降解过程有限速酶参与,柠檬酸钠、酒石酸钠会抑制该酶的活性,而NH4+对该酶有激活作用.关键词:黑曲霉;孟加拉红;色浆;脱色机理
一株黑曲霉(Aspergillus niger)对孟加拉红和水溶性色浆的脱色特性研究
Decoloring properties of Aspergillus niger to rose bengal and water-soluble color paste
【Abstract】 Aspergillus nigerisolated from soil by enrichment culture was chosen as the research object in this paper. The decoloring properties of Aspergillus niger to rose bengal and water-soluble color paste were studied in liquid culture with different carbon and nitrogen sources,pH value,and added metal ions. The decoloring effect was the best in a medium of bean sauce,with glycerol as carbon source,(NH4)2SO4 for nitrogen source,and initial pH of 6.0~8.0,but Cu2+ obviously inhibited the activity. By co... 更多
【关键词】 黑曲霉; 孟加拉红; 色浆; 脱色机理;
【Key words】 Aspergillus niger; rose bengal; color paste; decoloring mechanism;
【基金】 国家“十一五”科技支撑计划(No. 2008BADC4B08);科技部国际科技合作项目(No. 2010DFA62510);研究生创新基金项目(No.54040108003)~~ |
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