论文 Optimizing scale-up fermentation processes

细履平沙

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There are many aims associated with the optimization of fermentation processes. Optimization is expected to increase the yield of the final product but the process must be compliant with good manufacturing practices, the available equipment and the expected final scale of operation. Dealing with genetically modified microorganisms that overproduce recombinant protein has the advantage that the vast majority of the processes use only three different species, namely Escherichia coli,Saccharomyces cerevisiae and Pichia pastoris. Standard processes for each organism are described in textbooks and serve as a basis for the development of a tailored process. This article outlines the general philosophy that we have devised to ensure an efficient approach of scaling up fermentation processes for biopharmaceuticalpurposes, in a multidisciplinary environment.

郁郁栗林

发酵过程优化有许多目标，优化是为了提高最终结果的收益，但是过程必须和实际生产过程、可能的环境以及最终的预期目标较好的联系起来。对于基因工程菌来说，过量生产的重组蛋白的大部分进程只需要三个环，分别是剪切酶，异构酶和接合酶。每个有机物的标准过程是在检测书中提到的并且是剪切过程进行的基础。这篇文章描绘出我们为确保描述在各种不同的环境下目的产物的发酵过程设计的大体轮廓。

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good manufacturing practices, the available equipment and the expected final scale of operation-
good manufacturing practices----GMP
the available equipment -设备条件
the expected final scale of operation-预期最终生产规模